Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: Univariate logistic regression analysis for PTR and P/P PTR.

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques:

Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: Anti-HLA Antibodies distribution.

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques:

Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: The platelet transfusion response based on locus of HLA-I subgroups. The occurrence rate of overall (A) and primary (B) platelet transfusion efficacy was compared based on locus of HLA-I subgroups, and found that either anti-HLA-A, HLA-B or HLA-C antibody positive contribute to the ineffective to the platelet transfusion (all p < .05).

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques:

Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: Multivariate logistic regression identify anti-HLA-C autoantibodies as the risk factor for primary and persistent (P/P) platelet transfusion refractoriness. Factors with a univariate logistic regression result of p < .1 were included in the multivariate logistic regression analysis. The multivariate logistic regression analysis revealed that anti-HLA-I antibodies were an independent risk factor for PTR (2 A), but not in P/P PTR (2B); anti-HLA-C antibody was an independent risk factor for overall PTR (2 C), but not for P/P PTR (2D). Considering that anti-HLA-C antibody and anti-HLA-C autoantibody can be confounding factors, only anti-HLA-C autoantibody was selected for logistic regression analysis alongside anti-HLA-a and B antibodies. Result showed that no factors could predict the occurrence of PTR (2E), while anti-HLA-C autoantibody positive was the independent risk factor for P/P PTR (2 F). PTR: platelet transfusion refractoriness.

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques:

Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: Comparison of baseline characteristics in patients with or without anti-HLA-C autoantibodies in anti-HLA-C antibody positive population.

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques: Comparison

Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: Comparison Of the ABO-D matched and cross-matching platelet transfusions efficiency in patients with or without anti-HLA-C autoantibodies in anti-HLA-C antibody positive population. Significant differences were observed in both effective ordinary ( p < .001, 3 A) and cross-matching ( p = .017, 3B) platelet transfusion between patients with or without anti-HLA-C autoantibodies ( n = 6 vs. n = 26).

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques: Comparison

Journal: Annals of Medicine

Article Title: Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre

doi: 10.1080/07853890.2024.2446689

Figure Lengend Snippet: Details of patients with positive anti-HLA-C autoantibodies.

Article Snippet: Meanwhile, HLA antibodies were detected using HLA specific antibody detection kit (LABScreen ® , ONE LAMBDA), which utilize purified HLA antigen-coated microbeads to detect HLA antibodies by flow cytometry, and it defined mean fluorescent intensity (MFI) >500 as positive for an antibody [ ].

Techniques:

Journal: JHLT Open

Article Title: Belatacept-based immunosuppression in heart transplant recipients: A single center experience

doi: 10.1016/j.jhlto.2025.100277

Figure Lengend Snippet: The blue dotted lines separate two periods. The baseline period, in which antigens against HLA class I or II DSA developed within the first 85 days in both immunosuppression groups, and the follow-up period.

Article Snippet: Presence of Class I and II HLA antibodies in each patient case were identified using FlowPRA Class I and II Screening Tests (One Lambda, Inc., Canoga Park, CA) and LABScreenTM Single Antigen HLA Class I and LABScreen TM Single Antigen HLA Class II kits (One Lambda, Inc/Thermofisher).

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